Future Developments

TLC and HPLC are often looked at as competitive methods, but each has its own advantages. In HPLC, finding suitable separation parameters is frequently costly in terms of time and materials; therefore, a combination of the two by first optimizing the particular separation parameter with TLC is a step leading to considerable time-saving and cost for an analysis. TLC is suitable as a pilot technique for the

Table 34 Results from resolution of dansyl DL-amino acids

Dansyl dl-amino acid Pure l hRF from dl mixture Solvent system

d l NaCl-MeCN

Dansyl dl-amino acid Pure l hRF from dl mixture Solvent system

d l NaCl-MeCN

Phenylalanine

50

65

50

15 + 2

Valine

38

49

38

15 + 1.5

Tryptophan

23

34

23

20 + 0.5

Aspartic acid

55

67

55

15 + 2

61

70

61

18 + 2

52

60

52

15 + 1

30

52

30

20 + 0.5

Leucine

64

68

64

10 + 4 + 1 MeOH 9 + 3 + 0.5 MeOH

Norvaline

56

61

56

17 + 2 + 0.4 MeOH 16 + 2 + 0.25 MeOH

Temperature 25 $ 2°C. Solvent front, 10 cm. Time, 25-30 min. Visualization UV, 254 nm.

Temperature 25 $ 2°C. Solvent front, 10 cm. Time, 25-30 min. Visualization UV, 254 nm.

investigation of appropriate separation conditions, particularly because with TLC various phase systems can be checked at the same time without expensive apparatus. TLC will continue to serve as a useful method for daily routine control analyses to identify and determine the purity of a variety of compounds, including enantiomers, with ease and speed, and can be readily modified for new situations. A wide choice for separation conditions will always be available as various phase systems can be checked simultaneously.

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