Future Developments

The techniques for derivatizing and separating the standard amino acids in protein hydrolysates are mature and there is no significant room for improvement. Given the existence of quantitative derivatiz-ation protocols which proceed very rapidly, it is doubtful whether the development of on-column de-rivatization would constitute a significant advantage. Similarly, proteic amino acids can now be assayed in less than 10 min, so, given the availability of reliable automatic injectors, a reduction in analysis time is not of significant value.

Physiological samples may contain several hundred amino acids and these cannot, at present, all be resolved on any one single column. Frequently, however, only a subset is of interest. Thus, although the simultaneous derivatization and separation of all the proteic amino acids and as many as 50 of the more common nonproteic amino acids are now possible, it is likely that procedures targeted at specific subsets will remain important in specific contexts. The sensitivity of the FID detector is adequate for most analytical purposes but mass spectrometric detectors will remain important for specialized applications requiring femtomole sensitivity.

See also: III/Amino Acids: Liquid Chromatography; Thin-Layer (Planar) Chromatography. Amino Acids and Derivatives: Chiral Separations. Amino Acids and Peptides: Capillary Electrophoresis.

Solar Panel Basics

Solar Panel Basics

Global warming is a huge problem which will significantly affect every country in the world. Many people all over the world are trying to do whatever they can to help combat the effects of global warming. One of the ways that people can fight global warming is to reduce their dependence on non-renewable energy sources like oil and petroleum based products.

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