Further Reading

Chase HA (1984) Prediction of the performance of macropreparative affinity chromatography. Journal of Chromatography 297:179.

Chase HA (1988) Optimisation and scale-up of affinity chromatography. In Jennisen HP and MUller W (eds) Macromolecular Symposia 17. Basel: Hüthig & Wepf Verlag.

Chase HA (1988) Adsorption separation processes for protein purification. In Mizrahi (ed.) Downstream Processes: Equipment and Techniques. New York: Alan R. Liss.

Chase HA (1994) Purification of proteins from feedstocks containing particulate material by adsorption chromatog-raphy in expanded beds. Trends in Biotechnology 12: 296.

Dean PDG, Johnson WS and Middle FA (eds) (1985) Affinity Chromatography: A Practical Approach. Oxford: IRL Press.

Harrison RG (ed.) (1994) Protein Purification Process Engineering. New York: M. Dekker.

Janson JC and Ryden L (eds) (1997) Protein Purification: Principles, High Resolution Methods, and Application. New York: Wiley.

Kline T (ed.) (1993) Handbook of Affinity Chromatography. New York: Dekker.

Ladisch MR (ed.) (1990) Protein Purification: From Molecular Mechanisms to Large-scale Processes. Washington, DC: American Chemical Society.

Scopes RK (1994) Protein Purification: Principles and Practice. New York: Springer-Verlag.

Sofer GK and Hagel L (1997) Handbook of Process Chromatography: A Guide to Optimization, Scale-up, and Validation. San Diego: Academic Press.

Subramanian G (ed.) (1995) Process Scale Liquid Chromatography. New York: VCH.

Wheelwright SM (1991) Protein Purification: Design and Scale-up of Downstream Processing. Munich: Hanser Publishers.

inhibitors) usually by a multiplicity of non-covalent interactions. By contrast, the separation process in covalent chromatography does not require specific adsorptive binding and thus does not require knowledge of the structural determinants of the binding area of the component to be isolated. Instead, specificity relies on the nature of the chemical reaction of the chromatographic material with one or more components of a mixture. When complete specificity is

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