Further Reading

Anton K and Berger C (eds) (1998) Supercritical Fluid Chromatography with Packed Columns - Techniques and Applications. Chromatographic Sciences series. New York: Marcel Dekker. Grob K (1995) Review: development of the transfer techniques for on-line high-performance liquid chromatog-raphy - capillary gas chromatography. Journal of Chromatography A 703: 265. Hills D (ed.) (1998) Current Trends and Developments in Sample Preparation. LC GC International. Chester, UK: Advanstar Communications.

Niessen WMA and Tinke AP (1995) Review: liquid chromatography-mass spectrometry. General principles and instrumentation. Journal of Chromatography A 703: 37.

Poole CF and Poole SK (1991) Chromatography Today. Amsterdam: Elsevier.

A. E. Rodrigues, LSRE Faculty of Engineering,

University of Porto, Porto, Portugal

Copyright © 2000 Academic Press

New liquid chromatographic media have been developed in the last decade containing large pores for convective mass transport and smaller diffusive pores to provide adsorption capacity. These permeable packings are at the heart of perfusion chromatography, patented in 1991, leading to better column efficiency and speed of separation with applications in the rapid analysis of biological macro-molecules and preparative scale purification of proteins. This improved column efficiency is based on the concept of augmented diffusivity by convection, available from parallel development in the chemical reaction engineering area.

The analysis of column performance requires an extended van Deemter equation for the HETP (height equivalent to a theoretical plate) as a function of the superficial velocity. A methodology for obtaining basic data for design of perfusive chromatography is suggested involving elution chromatography of proteins in nonretained and frontal chromatography experiments.

Solar Panel Basics

Solar Panel Basics

Global warming is a huge problem which will significantly affect every country in the world. Many people all over the world are trying to do whatever they can to help combat the effects of global warming. One of the ways that people can fight global warming is to reduce their dependence on non-renewable energy sources like oil and petroleum based products.

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