Further Reading

Batta AK, Salen G, Rapole KR, Batta M, Earnest D and Alberts D (1998) Capillary gas chromatographic analysis of serum bile acids as the «-butyl ester-trimethylsilyl ether derivatives. Journal of Chromatography (B) 706: 337-341.

Child P, Aloe M and Mee D (1987) Separation and quanti-tation of fatty acids, sterols and bile acids in faeces by gas chromatography as the butyl ester-acetate derivatives. Journal of Chromatography 415: 13-26. Grundy SM, Ahrens Jr EH and Mietinnen TA (1965) Quantitative isolation and gas-liquid chromatographic analysis of total faecal bile acids. Journal of Lipid Research 6: 397-410. Kuksis A (1976) Gas chromatography of bile acids. In: Marinetti GV (ed.), Lipid Chromatographic Analysis, vol. 2, pp. 479-610. New York: Marcel Dekker. Sjovall J, Lawson AM and Setchell KDR (1985) Mass spectrometry of bile acids. In: Clayton RB (ed.) Methods in Enzymology 111: 63-113. New York: Academic Press.

chemical structure, all bile acids tend to form micelles and serve as natural detergents. In addition, bile acids are co-factors of pancreatic lipases and are pivotal for the digestion and absorption of fats and fat-soluble vitamins.

The primary bile acids cholic acid and cheno-deoxycholic acid are synthesized in the liver and during their enterohepatic circulation various modifications occur. Conjugation with the amino acids taurine or glycine as well as deconjugation, sulfation, glucuronidation and modification by intestinal

Liquid Chromatography bacteria lead to numerous secondary bile acid structures. The biliary and serum bile acid pattern as such as well as the detection of several secondary bile acids in serum can serve as disease markers and are of interest in therapeutic monitoring.

In healthy individuals the bile acid concentrations in the gall bladder and in duodenal bile are in the upper micromolar up to millimolar range. The determination of faecal bile acids is, however, not hampered by their overall concentration but by the complex pattern of primary and secondary derivatives as well as by the nature of the matrix. Bile acid patterns in serum, which are for healthy individuals in the upper nanomolar concentration range, are much harder to determine.

A number of gastrointestinal disorders and hepatic or biliary diseases, e.g. liver cirrhosis and hepatitis, lead to increased serum and urinary bile acid levels and result in significant changes in the bile acid pattern. Since bile acids are the major products of cholesterol metabolism, they play an important role in research and development of lipid lowering drugs and therapies.

This is the reason why simple, reproducible and sensitive methods for detection, separation and quan-titation of bile acid patterns in all kinds of biological matrices and body fluids are of increasing importance in the pharmaceutical industry as well as for clinical chemistry.

Solar Panel Basics

Solar Panel Basics

Global warming is a huge problem which will significantly affect every country in the world. Many people all over the world are trying to do whatever they can to help combat the effects of global warming. One of the ways that people can fight global warming is to reduce their dependence on non-renewable energy sources like oil and petroleum based products.

Get My Free Ebook


Post a comment