Further Reading

Anderson L (1991) Two-dimensional Electrophoresis: Operation of the ISO-DALT System. Rockville: Large Scale Biology Press.

Fountoulakis M and Lahm H-W (1998) Hydrolysis and amino acid composition analysis of proteins. Journal of Chromatography 826: 109.

Hames BD and Rickwood D (1990) Electrophoresis of Proteins: A Practical Approach. Oxford: IRL Press.

Humphery-Smith I, Cordwell SJ and Blackstock WP (1997) Proteome research: Complementarity and limitations with respect to the RNA and DNA worlds. Electrophor-esis 18: 1217.

Kleinert T (1990) Elektrophoretische Methoden in der Proteinanalytik. Stuttgart: Georg Thieme Verlag.

Righetti PG (1990) Immobilized pH Gradients: Theory and Methodology. Amsterdam: Elsevier.

Smith BJ (1997) Methods in Molecular Biology: Protein Sequencing Protocols, vol. 64. Totowa: Humana Press.

Walsh BJ and Herbert B (1998) Setting up Two-dimensional Gel Electrophoresis for Proteome Projects. http://rbams3115/Pages/2DPAGE/ABRFNews_2dpage.html

Westermeier R (1993) Electrophoresis in Practice. Weinheim: VCH Verlagsgesellschaft.

Wilkins MR, Williams KL, Appel RD and Hochstrasser DF (1997) Proteome Research: New Frontiers in Functional Genomics. Berlin: Springer.

well as the speed with which genome sequences are determined has generated huge amounts of information. This progress has boosted techniques, notably two-dimension polyacrylamide gel electrophoresis (2D-PAGE), enabling the analysis of a proteome consisting of all the proteins expressed by a genome. Such analyses give information on the effector molecule itself, namely the protein, and take into account highly sophisticated mechanisms regulating gene expression. 2D-PAGE is the most powerful tool to separate a multitude of polypeptides that are contained in a single biological sample. Various procedures have been described to separate proteins according to biophysical parameters. In 1975, O'Farrel, Klose and Scheele described optimized 2D procedures in which proteins were denatured and separated by electrophoresis on polyacrylamide gel. The first gel dimension comprised a separation according to the protein charge by isoelectric focusing,

Solar Panel Basics

Solar Panel Basics

Global warming is a huge problem which will significantly affect every country in the world. Many people all over the world are trying to do whatever they can to help combat the effects of global warming. One of the ways that people can fight global warming is to reduce their dependence on non-renewable energy sources like oil and petroleum based products.

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