Further Reading

B+g-Hansen TC, Bjerrum OJ and Ramlau J (1975) Detection of biospecific interaction during the first dimension electrophoresis in crossed immunoelectrophoresis. Scandinavian Journal of Immunology 4 (Suppl. 2): 141.

Breborowicz J and Mackiewicz A (eds) (1992) Affinity Electrophoresis: Principles and Application. Boca Raton: CRC Press.

Dolnik V (1997) Capillary zone electrophoresis of proteins. Electrophoresis 18: 2353.

Grabar P and Williams CA (1953) Methode permettent l'etude conjugee des propietes electrophoretiques et im-munochimiques d'un me lange de prote ines. Application au serum sagnuin. Biochimica Biophysica Acta 10: 193.

Helenius A and Simons K (1977) Charge shift electrophoresis: simple method for distinguishing between am-phiphilic and hydrophilic proteins in detergent solution. Proceedings of the National Academy of Science of the USA 74: 529.

Laurell CB (1966) Quantitative estimation of proteins by electrophoresis in agarose gel containing antibodies. Analytical Biochemistry 15: 45.

Ouchterlony O (1948) In vitro method for testing the toxin-producing capacity of diphtheria bacteria. Acta Patho-logica Microbiologics. Scandinavica 25: 186.

Ouchterlony O and Nilsson LA (1986) Immunodiffusion and immunoelectrophoresis. In: Weir M, Herzenerg LA, Blackwell C and Herzenberg LA (eds) Handbook of Experimental Immunology 4th edn, vol. 1. Immunochemisty, Ch. 32. Oxford: Blackwell Scientific Publications.

Ressler N (1960) Two-dimensional electrophoresis of proteins antigens with an antibody containing buffer. Clinica Chimica Acta 5: 795.

Uriel J (1971) Color reactions for identification of antigen-antibody precipitates in gels. In: Williams CA and Chase MW (eds) Methods in Immunology and Immuno-chemistry, vol. III, p. 294. New York: Academic Press.

Williams CA (1971) Immunoelectrophoretic analysis. In: Williams CA and Chase MW (eds) Methods in Immunology and Immunochemistry, vol. III, p. 235. New York: Academic Press.

attained by amphoteric species (mostly proteins and peptides) along a pH gradient under the influence of an electric field. Due to a continuous balancing of diffusion away from the pi (isoelectric point) and pi-driven electric forces, extremely sharp zones are obtained, characterized by a very high resolving power. This article will consider conventional isoelec-tric focusing (IEF) in soluble, amphoteric buffers; and immobilized pH gradients (IPG) in insolubilized,

Solar Panel Basics

Solar Panel Basics

Global warming is a huge problem which will significantly affect every country in the world. Many people all over the world are trying to do whatever they can to help combat the effects of global warming. One of the ways that people can fight global warming is to reduce their dependence on non-renewable energy sources like oil and petroleum based products.

Get My Free Ebook

Post a comment