From the First to the Second Dimension

Transfer of the first-dimension spaghetti-like gels after isoelectric focusing in the presence of am-

pholytes used to be a manipulation challenge for beginners in the field of 2D-PAGE. Extrusion of the gels from the glass tubes was difficult, and the gels frequently broke into several pieces. With the use of IPG strips, which are deposited on a plastic backing material, the transfer is easy. Practically, after the first dimension run, the strips are equilibrated in buffers containing SDS in order to maintain proteins in solution and to reduce -S-S- bonds. Subsequently, strips are placed over SDS gels that may or may not contain a stacking gel.

Solar Panel Basics

Solar Panel Basics

Global warming is a huge problem which will significantly affect every country in the world. Many people all over the world are trying to do whatever they can to help combat the effects of global warming. One of the ways that people can fight global warming is to reduce their dependence on non-renewable energy sources like oil and petroleum based products.

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