Effect of pH of the Mobile Phase

For weak acid and basic analytes the mobile phase pH value influences the degree of ionization and con

Figure 6 Retention factor for (A) phenylalanine and (B) naphthalene-2-sulfonicacid as a function of the methanol concentration (0) in the phosphate buffer (pH 2.1 and constant ionic strength 175 mM Na #) in the mobile phase in the absence (□) and presence (A) of 5 mM sodium octylsulfonate pairing ion. (Reproduced with permission from Bartha A and Stahlberg J (1994) Journal ofChromatogra-phyA 668: 255.)

Figure 6 Retention factor for (A) phenylalanine and (B) naphthalene-2-sulfonicacid as a function of the methanol concentration (0) in the phosphate buffer (pH 2.1 and constant ionic strength 175 mM Na #) in the mobile phase in the absence (□) and presence (A) of 5 mM sodium octylsulfonate pairing ion. (Reproduced with permission from Bartha A and Stahlberg J (1994) Journal ofChromatogra-phyA 668: 255.)

sequently its retention. In a reversed-phase system, with no ion pair reagent added, the retention factor kB is the weighted sum of the retention factor for the charged, kiB, and the uncharged, kuB, species, respectively:

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Solar Panel Basics

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