Drug Sample Preparation Techniques

A reliable analytical method is achieved with efficient sample preparation, adequate chromatographic separation, and a sensitive detection technique. Detailed and exact guidelines exist for the validation of bi-oanalytical methods, which meet requirements agreed to by the Food and Drug Administration. The sample preparation step is an important component of each overall bioanalytical method, as it

• often concentrates an analyte to improve its limits of detection,

• removes unwanted matrix components that can cause interferences upon analysis, thus improving method specificity, and

• frees the analyte from matrix components so that it can be placed into a solvent suitable for injection into the chromatographic system.

Liquid/liquid extraction

A common sample preparation procedure used to isolate drug analytes from biological matrices is liquid/liquid extraction (LLE). It is quite effective at removing salts and proteins; water-soluble endogenous components often remain in the aqueous phase.

Solar Panel Basics

Solar Panel Basics

Global warming is a huge problem which will significantly affect every country in the world. Many people all over the world are trying to do whatever they can to help combat the effects of global warming. One of the ways that people can fight global warming is to reduce their dependence on non-renewable energy sources like oil and petroleum based products.

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