Development of Dye Ligands and Dye Affinity Adsorbents

The originally exploited dyes were commercial textile chlorotriazine aromatic polysulfonated molecules

Eldjarn L and Jellum E (1963) Organomercurial polysaccharide - a chromatographic material for the separation and isolation of SH-proteins. Acta Chemica Scandina-vica 17: 2610-2621.

Evans B and Shaw E (1983) Inactivation of cathepsin B by active-site directed disulRde exchange. Application in covalent affinity chromatography. Journal of Biological Chemistry 258: 10227-10232.

Hillson DA (1981) Resolution of thiol-containing proteins by sequential-elution covalent chromatography. Journal of Biochemical and Biophysical Methods 4: 101-111.

Lozinskii VI and Rogozhin SV (1980) Chemospecific (covalent) chromatography of biopolymers. Russian Chemical Reviews 49: 460-472.

which when attached to appropriate supports, usually bearing hydroxyl groups, yield dye affinity adsorbents. The range of shades of commercial dyes derives primarily from anthraquinone, azo and phthalocyanine chromophores bonded to suitable reactive functions such as triazinyl and other mainly polyhalogenated heterocyclics. Anthraquinone dyes produce blue and the phthalocyanines turquoise shades. Green dyes contain mixed anthraquinone-stilbene, anthraquinone-azo or phthalocyanine-azo structures, whereas most other shades are derived from the azo class.

Unlike most biological affinity adsorbents, the stability of dye affinity adsorbents is usually limited only by the support itself. Dyes offer clear advantages over biological ligands, in terms of economy, ease of immobilization, safety, stability and adsorbent capacity. The main drawback of textile dyes is their moderate selectivity during the protein-binding process. In spite of this, the overall size, shape and distribution of ionic and hydrophobic groups enable dyes to interact with the binding sites(s) of proteins sometimes fairly specifically, as for example, with the nucleotide-binding site of several dehydro-genases, kinases, and several nucleotide-recognizing enzymes. The dye-protein interaction should not be compared to a simple ion exchange type since binding is frequently possible at pHs greater than the pi of the targeted protein. Furthermore, dissociation of the dye-protein complex is often achieved specifically by competing ligands, suggesting interaction with the protein at discrete sites. The view is supported by chromatographic, kinetic, inactivation, affinity labelling and spectra difference studies.

The last few years have seen a novel approach for tackling the problem of dye selectivity, signalling the

Solar Panel Basics

Solar Panel Basics

Global warming is a huge problem which will significantly affect every country in the world. Many people all over the world are trying to do whatever they can to help combat the effects of global warming. One of the ways that people can fight global warming is to reduce their dependence on non-renewable energy sources like oil and petroleum based products.

Get My Free Ebook

Post a comment