Determination of Elution Volumes

The absolute flow rate can be obtained by measuring the time to fill a calibrated flask or by weighing the solvent passing the system in a defined time.

The knowledge of the absolute flow rate is, however, not completely necessary as long as flow rate variations are compensated by using an internal standard. The corrected flow rate is obtained from the ratio of the elution times of this standard peak. Such a correction only works well, if the flow rate is sufficiently constant within the entire chromatogram.

Solar Panel Basics

Solar Panel Basics

Global warming is a huge problem which will significantly affect every country in the world. Many people all over the world are trying to do whatever they can to help combat the effects of global warming. One of the ways that people can fight global warming is to reduce their dependence on non-renewable energy sources like oil and petroleum based products.

Get My Free Ebook


Post a comment