Derivatization

Figure 1 Chemical derivatization of an analyte using 6-aminoquinoyl-A/-hydroxysuccinimidyl carbamate (6-AQC) reagent. This is a homogeneous reaction that occurs in solution. The 6-AQC degrades with water to form 6-aminoquinolineand A/-hydroxysuccinimide, with the release of carbon dioxide.

analyte or by-products of the reagent, in a state suitable for direct LC injection.

Other approaches to derivatization involve the use of photochemical reactions, usually performed online after the separation occurs, which convert the starting analyte into one or more derivatives with improved detection properties (ultraviolet (UV), fluorescent (FL), electrochemical (EC), etc). This does not introduce excess derivatizing reagent, reagent byproducts or hydrolysis products, since the reagent itself is light rather than a chemical. Such approaches have become popular in LC applications. It is also possible to utilize electrochemistry to perform de-

rivatizations in LC, as well as microwave digestion, immobilized enzyme reactors, pH alteration of the mobile phase after the separation, etc. Table 1 summarizes the most commonly utilized derivatization techniques described in LC other than simple, chemical reactions.

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