Data Management

Many investigators have analysed their 2D gels by holding two, sometimes three gels together towards a light source, and tried to identify differences between them (Figure 4). However, analysis of a multitude of 2D gels, with its bulk of information is greatly

Figure 4 Analysis of 2D gels. 2D gels of human platelets from a single blood donor (pH 3.5-10 carrier ampholyte gradient). (A) Platelets stored with leukocytes, 1 day after collection; (B) platelets stored in the presence of leukocytes, 7 days after collection; (C) platelets stored in the absence of leukocytes, 7 days after collection. Without computerized analysis, it is not possible to draw a definitive conclusion from the comparison of this set of gels. Reproduced with permission from Sarraj-Reguieg A, Tissot JD, Hochstrasser DF, von FliednerV, Bachmann F and Schneider P (1993) Effect of prestorage leukocyte reduction on proteins of platelets obtained by apheresis. Vox Sanguinis 65: 279.

Figure 4 Analysis of 2D gels. 2D gels of human platelets from a single blood donor (pH 3.5-10 carrier ampholyte gradient). (A) Platelets stored with leukocytes, 1 day after collection; (B) platelets stored in the presence of leukocytes, 7 days after collection; (C) platelets stored in the absence of leukocytes, 7 days after collection. Without computerized analysis, it is not possible to draw a definitive conclusion from the comparison of this set of gels. Reproduced with permission from Sarraj-Reguieg A, Tissot JD, Hochstrasser DF, von FliednerV, Bachmann F and Schneider P (1993) Effect of prestorage leukocyte reduction on proteins of platelets obtained by apheresis. Vox Sanguinis 65: 279.

facilitated by the use of computer-based data processing. The improvements in image acquisition and image analysis allow clear spot detection, background subtraction, spot matching and database construction. Furthermore, interpretation of 2D-PAGE images is facilitated by statistical methods, artificial intelligence and machine-learning programs. Ascendant hierarchical classification sorts the image into meaningful groups. The use of correspondence analysis and ascendant hierarchical clustering allows identification of new, potentially important proteins. Many database of 2D gel master images are accessible through the World Wide Web (Internet sites can be found at the following addresses: http://www.ex-pasy.ch/ch2d/2d-index.html or http://www-lmmb. ncifcrf.gov/ABRF97//abrf3.html). It is also possible to compare 2D gels from various laboratories, or 2D gels with masters, on the World Wide Web by using the flicker created by P.F. Lemkin (accessible at http://www-lecb.ncifcrf.gov/flicker/).

Solar Panel Basics

Solar Panel Basics

Global warming is a huge problem which will significantly affect every country in the world. Many people all over the world are trying to do whatever they can to help combat the effects of global warming. One of the ways that people can fight global warming is to reduce their dependence on non-renewable energy sources like oil and petroleum based products.

Get My Free Ebook


Post a comment