Coupling HPLC with Planar Chromatography

Combining separation techniques that utilize different mechanisms to a multidimensional approach increases the potential of the individual techniques by an order of magnitude. HPLC in reversed-phase mode is one of the most powerful separation techniques available for nonvolatile substances. Online coupling of HPLC with a multiple development system using normal-phase chromatography results in peak capacities of around 500.

A device for mass transfer from HPLC to planar chromatography consists of a sample spray-on device that is connected to the column outlet. Since the maximum quantity of liquid that can be sprayed on a silica layer without washing it away is limited to 10-60 iLmin"1, depending on the mobile phase, a microbore HPLC unit is the most appropriate for this technique.

Solar Panel Basics

Solar Panel Basics

Global warming is a huge problem which will significantly affect every country in the world. Many people all over the world are trying to do whatever they can to help combat the effects of global warming. One of the ways that people can fight global warming is to reduce their dependence on non-renewable energy sources like oil and petroleum based products.

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