Countercurrent Chromatography

dissolving the suitable chiral selector in the liquid stationary phase.

In the past various hydrostatic CCC systems, such as droplet CCC, rotation locular CCC (RLCCC) and centrifugal partition chromatography (CPC), have been used for the separation of chiral compounds. None of those techniques, however, is considered satisfactory for preparative purposes in terms of sample size, resolution and/or separation time. Recently, the hydrodynamic CCC system termed high speed CCC has remarkably improved the technique so that highly efficient separations can be achieved in a short elution time. This technique has been successfully applied to the separation of racemates using a Pirkle-type chiral selector. In this method analytical (milligram) and preparative (gram) separations can be performed simply by adjusting the amount of chiral selector in the liquid stationary phase in the standard separation column. Gram quantities of en-antiomers can be obtained by pH-zone-refining CCC, a new preparative separation technique recently developed for the separation of ionizable compounds (see pH-zone-refining CCC). In addition, the present method allows computation of the formation constant of the chiral-selector complex, one of the most important parameters for studies on the mechanism of enantioselectivity.

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