Coomassie Brilliant Blue

The most popular general protein-staining procedures following gel electrophoresis are based on the use of the non-polar, sulfated triphenylmethane Coomassie stains, developed for the textile industry, Coomassie Brilliant Blue (CBB) R-250 is most often used and requires an acidic medium for electrostatic interaction between the dye molecules and the amino groups of proteins. Staining is usually carried out using 0.1% w/v CBB R-250 in the same acid methanol solution used for fixation (10% acetic acid, 45% methanol). Depending on gel thickness and poly-acrylamide concentration, staining can take from 30 min to several hours. In practice, it is often convenient to stain the gel overnight and then destain it by several changes in the same acid methanol solution until intense blue protein zones can be seen against a clear background. This method is able to detect a minimum of around 100 ng protein per band (Figure 1), so that for complex mixtures containing several hundred components, it is necessary to load relatively high amounts of total protein ( > 50 |g).

Solar Panel Basics

Solar Panel Basics

Global warming is a huge problem which will significantly affect every country in the world. Many people all over the world are trying to do whatever they can to help combat the effects of global warming. One of the ways that people can fight global warming is to reduce their dependence on non-renewable energy sources like oil and petroleum based products.

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