Conclusions

Because it is a support-free partition system, HSCCC has an important advantage over other chromato-graphic methods in that it eliminates various com-

Figure 12 Effect of TFA concentration on the partition coefficients of CL components. (Reproduced with permission from Ikai Y etal. (1998) and Oka H etal. (1998).)

plications such as adsorptive loss and deactivation of samples as well as contamination from the solid support. As shown by our examples, HSCCC can isolate various components from a complex mixture of antibiotics by carefully selecting the two-phase solvent system to optimize the partition coefficient (K) of the target component(s). Compared with CCD and other countercurrent extraction methods, HSCCC can yield higher partition efficiencies in a shorter elution time. The HSCCC system can also be applied to microanalytical-scale separations without excessive dilution of samples. We believe that HSCCC is an

Figure 13 HSCCC separation of commerical CL. Apparatus, HSCCC-1A; revolution, 800 rpm; solvent system, n-butanol/ 0.04 mol L"1 TFA aqueous solution (1 :1); mobile phase, lower aqueous phase; flow rate, 2.0 mL min"1; detection, 220 nm. (Reproduced with permission from Ikai Y et al. (1998) and Oka H etal. (1998).)

Figure 12 Effect of TFA concentration on the partition coefficients of CL components. (Reproduced with permission from Ikai Y etal. (1998) and Oka H etal. (1998).)

Figure 13 HSCCC separation of commerical CL. Apparatus, HSCCC-1A; revolution, 800 rpm; solvent system, n-butanol/ 0.04 mol L"1 TFA aqueous solution (1 :1); mobile phase, lower aqueous phase; flow rate, 2.0 mL min"1; detection, 220 nm. (Reproduced with permission from Ikai Y et al. (1998) and Oka H etal. (1998).)

Figure 14 HPLC analysis of CL components of HSCCC fractions. (A) CL-A (Fraction 5); (B) CL-B (Fraction 3). (Reproduced with permission from Oka H etal. (1998).)

ideal method for separation and purification of antibiotics.

See also: II/Chromatography: Countercurrent Chromatography and High-Speed Countercurrent. Chromatography:

Instrumentation. Chromatography: Liquid: Countercurrent Liquid Chromatography. III/Antibiotics: Liquid Chromatography. Supercritical Fluid Chromatography.

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