The choice of FFF separation techniques for purification of cell or cell organelle populations is influenced by their possible elution modes. Micron sized species eluted under the steric hyperlayer model can be separated using sedimentation techniques if their density differs from that of the carrier phase medium. This is also possible for 'nonbuoyant' sub-micron sized cell organelle species eluted according to the 'Brownian' elution mode.

If species are to be separated according to their size, FFF techniques that use a flow generated external field are preferred. If differences in surface characteristics are important, electrical-based FFF techniques should be chosen. For large sample volume preparations, SPLITT techniques are useful, whatever the external field. The major technical goal in cell separation is not the set-up of the separator, but the construction of a chain of knowledge. The separator is designed according to the sample characteristics as well as the mode of operation. Cleaning and sterilization procedures, and detection and viability procedures, are linked to the nature of the material to be purified. This 'biotechnological' process is of major importance for FFF separations in the life sciences.

See also: Il/Particle Size Separation: Field Flow Fractionation: Electric Fields; Theory and Instrumentation of Field Flow Fractionation. Ill/Colloids: Field Flow Fractionation. Polymers: Field Flow Fractionation. Proteins: Field Flow Fractionation. Ill/Catalyst studies: Chromatography: Isolation: Magnetic Techniques.

Solar Panel Basics

Solar Panel Basics

Global warming is a huge problem which will significantly affect every country in the world. Many people all over the world are trying to do whatever they can to help combat the effects of global warming. One of the ways that people can fight global warming is to reduce their dependence on non-renewable energy sources like oil and petroleum based products.

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