Conclusions

IP-TLC can be used to advantage for the modulation of the chromatography of acidic and basic analytes on a range of stationary phases. The way in which the reagents are used (i.e. in the mobile phase or impregnated on to the plate) depends on the structure of the reagent itself and the nature of the stationary phase. Probably the most important factors controlling the magnitude of the effect obtained when using an IP reagent in planar chromatography are the structure of the reagent (with long chain reagents giving the biggest effects) and the concentration of reagent employed. In contrast to HPLC, pH has relatively little effect in many IP-TLC systems.

See also: N/Chromatography: Liquid: Ion Pair Liquid Chromatography. Chromatography: Thin-Layer (Planar): Modes of Development: Forced Flow, Over-pressured Layer Chromatography and Centrifugal. III /Acids: Thin-Layer (Planar) Chromatography. Bases: Thin-Layer (Planar) Chromatography. Chiral Separations: Ion-Pair Chromatography; Thin-Layer (Planar) Chromatography. Impregnation Techniques: Thin-Layer (Planar) Chromatography.

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