Conclusions

From the foregoing it can be concluded that hydro-phobic interaction chromatography is one of the very basic separation methods in classical biochemistry. A great deal of information on the mechanisms involved in the method has been obtained and it appears that the critical hydrophobicity method for the optimization of hydrophobic supports offers a rational approach to the purification of proteins. The only drawback is that such hydro-phobic gel series are not commercially available so that the application of this method necessitates experience in the synthesis of alkyl agaroses and the quantification of immobilized residues. Methodological investments of this types thus pose the 'high-energy barrier' to a more widespread and successful application of hydrophobic interaction chromatography in enzymology and protein chemistry.

See also: I/Affinity Separation. III/Affinity Separation:

Liquid Chromatography. Glycoproteins: Liquid Chromatography. pH-Zone Refining Countercurrent Chromatography: High Speed Countercurrent Chromatography. Polymers: Field Flow Fractionation. Appendix 1/Essen-tial Guides for Isolation/Purification of Enzymes and Proteins.

Solar Panel Basics

Solar Panel Basics

Global warming is a huge problem which will significantly affect every country in the world. Many people all over the world are trying to do whatever they can to help combat the effects of global warming. One of the ways that people can fight global warming is to reduce their dependence on non-renewable energy sources like oil and petroleum based products.

Get My Free Ebook


Post a comment