Conclusions

The choice of FFF separation techniques for purification of cell or cell organelle populations is influenced by their possible elution modes. Micron sized species eluted under the steric hyperlayer model can be separated using sedimentation techniques if their density differs from that of the carrier phase medium. This is also possible for 'nonbuoyant' sub-micron sized cell organelle species eluted according to the 'Brownian' elution mode.

If species are to be separated according to their size, FFF techniques that use a flow generated external field are preferred. If differences in surface characteristics are important, electrical-based FFF techniques should be chosen. For large sample volume preparations, SPLITT techniques are useful, whatever the external field. The major technical goal in cell separation is not the set-up of the separator, but the construction of a chain of knowledge. The separator is designed according to the sample characteristics as well as the mode of operation. Cleaning and sterilization procedures, and detection and viability procedures, are linked to the nature of the material to be purified. This 'biotechnological' process is of major importance for FFF separations in the life sciences.

See also: Il/Particle Size Separation: Field Flow Fractionation: Electric Fields; Theory and Instrumentation of Field Flow Fractionation. Ill/Colloids: Field Flow Fractionation. Polymers: Field Flow Fractionation. Proteins: Field Flow Fractionation. Ill/Catalyst studies: Chromatography: Isolation: Magnetic Techniques.

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