The purification of proteins on immobilized metal ions is both effective and versatile. As well as a long standing role in the isolation of proteins with naturally available histidine residues, it has now become an everyday method for the isolation of 6His-tagged recombinant proteins. Recent reviews on the separation of phosphoproteins on immbolized Fe3 + indicate continued interest in such applications and no doubt additional uses will be found in the future.

See also: I/Affinity Separation. II/Affinity Separation:

Theory and Development of Affinity Chromatography. III/Enzymes: Liquid Chromatography; Proteins: HighSpeed Countercurrent Chromatography. Appendix 1/ Essential Guides for Isolation/Purification of Enzymes and Proteins.

Solar Panel Basics

Solar Panel Basics

Global warming is a huge problem which will significantly affect every country in the world. Many people all over the world are trying to do whatever they can to help combat the effects of global warming. One of the ways that people can fight global warming is to reduce their dependence on non-renewable energy sources like oil and petroleum based products.

Get My Free Ebook

Post a comment