Concluding Remarks

The purification of bacteriophages by centrifugation is a routine procedure. The most difficult requirement is the obtaining of centrifuges. Challenges are encountered primarily when a bacteriophage is unstable during exposure to the conventional conditions of centrifugation. A further challenge is encountered when bacteriophage particles are purified for the purpose of high resolution analysis of structure. This latter challenge is heterogeneity of bacteri-ophage particles that appear homogeneous when fractionated by centrifugation. Nondenaturing gel electrophoresis can reveal heterogeneity. However, nondenaturing gel electrophoresis has not been useful for preparative fractionation. Thus, a challenge for the future is the development of preparative procedures of nondenaturing gel electrophoresis. This last challenge would be met by development of a procedure of continuous-flow gel electrophoresis. Continuous-flow preparative gel electrophoresis is possible, in theory. Hopefully, it will be achieved in practice.

See also: II/Electrophoresis: Agarose Gels. Centrifugation: Theory of Centrifugation.

Solar Panel Basics

Solar Panel Basics

Global warming is a huge problem which will significantly affect every country in the world. Many people all over the world are trying to do whatever they can to help combat the effects of global warming. One of the ways that people can fight global warming is to reduce their dependence on non-renewable energy sources like oil and petroleum based products.

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