Concluding Comments

The ease with which specificity and selectivity can be provided in covalent chromatography involving attachment via thiol groups, together with the advantages due to the mild conditions required for attachment, elution and reactivation of the gel, account for the outstanding success of versions of the technique involving thiol-disulfide interchange, particularly those using 2-pyridyl disulfide sites. Attachments via other protein side chains are generally less satisfactory in these respects and have been used only to a limited extent. Often covalent chromatography has been used at a relatively late stage in the purification process but the successful isolation of bovine mercaptalbumin from crude extracts reported by Carlsson and Svenson in 1974 suggests that this approach should be considered in other cases. Some of the applications listed in Table 2 can be applied to non-thiol-containing proteins by the introduction of an auxiliary thiol group, e.g. by use of the valuable heterobifunctional reagent, N-succinimidyl-3-(2'-py-ridyl disulfanyl) propanoate, introduced by Carlsson, Drevin and Axen in 1978 or by site-directed mutagenesis.

Solar Panel Basics

Solar Panel Basics

Global warming is a huge problem which will significantly affect every country in the world. Many people all over the world are trying to do whatever they can to help combat the effects of global warming. One of the ways that people can fight global warming is to reduce their dependence on non-renewable energy sources like oil and petroleum based products.

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