Computer Controlled Multiple Development of Planar Chromatograms

Automated multiple development, more particularly when performed as a gradient technique, achieves the maximum resolution feasible within the limited separation distance available on a HPTLC plate. In terms of peak capacity it compares with HPLC while retaining the inherent benefits of planar chromatography. The automated multiple developing system (AMD; Figure 4) serves for this type of chromatogram development. A step gradient of decreasing eluotropic strength and changing selectivity is created and each development proceeds to a higher migration distance than the one before. A typical standard gradient changes the developing solvent from methanol to dichloromethane to hexane in 25 steps over a final migration distance of 65 mm. Between each step the plate is dried under vacuum. The software allows the composition of developing solvent to be specified as well as the developing distance for each step. Solvent volumes are measured with syringes and migration distance of the solvent front is measured with sensors. Preconditioning through the gas phase prior to development is possible.

Solar Panel Basics

Solar Panel Basics

Global warming is a huge problem which will significantly affect every country in the world. Many people all over the world are trying to do whatever they can to help combat the effects of global warming. One of the ways that people can fight global warming is to reduce their dependence on non-renewable energy sources like oil and petroleum based products.

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