Comprehensive Equation Describing Electrophoretic Mobility of Proteins Migrating in a Linear PA Gradient

As explained above, the velocity with which a protein migrates in a linear PA gradient gel depends on its apparent free electrophoretic mobility times a retardation factor (eqn [27]):

where v is the migration velocity (m s"1), T (%) is the PA concentration which the migrating protein of mobility vhas reached and Tmax (%) represents the exclusion limit of the migrating protein.

Since U = / x E"1 (m2V"1s"1), it follows that:

vxE"1 = Ux[1-(TxTm1x)]i (m V"1 s"1) [53]

U is defined by eqn [32] as equivalent to:

with the definitions given above.

Using all this information, a complete description of the electrophoretic mobility of proteins migrating in a linear PA gradient gel can then be given by the equation:

vxE"1 = (Zx g) x (6 xrexy xRS)"1 x (X1(kxRs))

x (1 + (k xRs))"1 x [1 — (TxTJ)]' (m2(Vs)"1)

with the definitions as given above.

Solar Panel Basics

Solar Panel Basics

Global warming is a huge problem which will significantly affect every country in the world. Many people all over the world are trying to do whatever they can to help combat the effects of global warming. One of the ways that people can fight global warming is to reduce their dependence on non-renewable energy sources like oil and petroleum based products.

Get My Free Ebook

Post a comment