Choice of Electrolyte

The choice of electrolyte is important and will influence the separation. However, due to the low solubility of many electrolytes in organic solvents, it can be difficult to find a suitable electrolyte. The more polar solvents, like MeOH, DMSO, formamide, N-methyl-formamide and N,N-dimethylformamide, possess a good solvating power towards the electrolytes commonly used in NACE. So far, ammonium acetate has been the most frequently used electrolyte in NACE systems and acetic acid or sodium acetate have often been used in combination with ammonium acetate in order to adjust the acid-base properties of the electrophoresis medium. Quaternary ammonium salts have also been used a number of times with success, e.g. in the separation of phenols and car-boxylic acids. More rarely, Tris, magnesium acetate, citric acid, formic acid, triSuoroacetic acid and methanesulfonic acid have been used.

When coupling CE to mass spectrometry (MS), it is an advantage to choose a volatile electrolyte, e.g. ammonium acetate, in order to limit background noise or cluster ion formation.

Solar Panel Basics

Solar Panel Basics

Global warming is a huge problem which will significantly affect every country in the world. Many people all over the world are trying to do whatever they can to help combat the effects of global warming. One of the ways that people can fight global warming is to reduce their dependence on non-renewable energy sources like oil and petroleum based products.

Get My Free Ebook

Post a comment