Choice of Electrolyte

The choice of electrolyte is important and will influence the separation. However, due to the low solubility of many electrolytes in organic solvents, it can be difficult to find a suitable electrolyte. The more polar solvents, like MeOH, DMSO, formamide, N-methyl-formamide and N,N-dimethylformamide, possess a good solvating power towards the electrolytes commonly used in NACE. So far, ammonium acetate has been the most frequently used electrolyte in NACE systems and acetic acid or sodium acetate have often been used in combination with ammonium acetate in order to adjust the acid-base properties of the electrophoresis medium. Quaternary ammonium salts have also been used a number of times with success, e.g. in the separation of phenols and car-boxylic acids. More rarely, Tris, magnesium acetate, citric acid, formic acid, triSuoroacetic acid and methanesulfonic acid have been used.

When coupling CE to mass spectrometry (MS), it is an advantage to choose a volatile electrolyte, e.g. ammonium acetate, in order to limit background noise or cluster ion formation.

Solar Panel Basics

Solar Panel Basics

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