Chiral Mobile Phases

CMPs permit the use of conventional stationary phases and show fewer detection problems than CSPs or CCPs. However, high cost chiral selectors (i.e. y-cyclodextrin) are certainly not advisable for TLC.

Enantiomer separations have been achieved using chiral mobile phases in both normal and reversed-phase chromatography. The first technique employs silica gel and, mostly, Diol F254 HPTLC plates (Merck) and, as chiral selectors, d-galacturonic acid, N-carbobenzoxy(CBZ)-l-amino acids or peptides, 1R-( — )-ammonium-10-camphorsulfonate and 2-O-[(R)-2-hydroxypropyl)]-ft-cyclodextrin.

Most separations have been obtained by reversed-phase chromatography on hydrophobic silica gel with ft-cyclodextrin (ft-CD) and its derivatives, bovine

N COOH

C10H21

Figure 1 Structure of (2S,4R,2RS)-N-(2-hydroxydodecyl)-4-hydroxyproline.

serum albumin (BSA) and the macrocyclic antibiotic vancomycin as chiral agents.

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