Carbenicillin Sulbenicillin and Ticarcillin

These epimeric, di-anionic ^-lactams are similar in physicochemical properties and are analysed under very similar conditions. For analysis of carbenicillin (Figure 3, structure 7), plasma and urine samples are loaded onto an anion exchange solid-phase extraction column. Carbenicillin epimers are eluted with 10% lithium chloride-methanol (3 : 2) and injected into an HPLC. Analysis is on an ODS column with a mobile phase consisting of 0.05 mol L_1 ammonium acetate-methanol (9 : 1). Carbenicillin

Figure 4 Chromatogram of human plasma spiked with carbenicillin. Five hundred microlitres of human plasma was spiked with 20 |L of an aqueous solution of carbenicillin (5.2 mg mL~1). R, R-epimer, S, S-epimer.

epimers are detected at 254 nm. The epimers are resolved to the baseline with the R-epimer being eluted prior to the S-epimer (Figure 4).

Similar methods can be applied for the analysis of sulbenicillin (Figure 3, structure 8) and ticarcillin (Figure 3, structure 10). For sulbenicillin, the mobile phase consists of 0.05 mol L_1 phosphate buffer (pH 7.0)-methanol (8 : 1). The epimers are resolved to the baseline with the S-epimer being eluted faster than the R-epimer. The detection limit is 0.5 |g mL_1 for each epimer. For ticarcillin, the mobile phase consists of 0.05 mol L_1 phosphate buffer (pH 7.0)-methanol (12 : 1). The epimers are resolved to the baseline with the R-epimer being eluted faster than the S-epimer.

Solar Panel Basics

Solar Panel Basics

Global warming is a huge problem which will significantly affect every country in the world. Many people all over the world are trying to do whatever they can to help combat the effects of global warming. One of the ways that people can fight global warming is to reduce their dependence on non-renewable energy sources like oil and petroleum based products.

Get My Free Ebook


Post a comment