Capillary Electrophoresis

The feasibility of using imprinted polymers as selective matrices for affinity capillary electrophoresis and capillary electrochromatography has been demonstrated. Owing to the difficulty in packing ground polymer particles or polymer beads into microbore capillaries, an in-situ polymerization seems better suited for this application. Imprinted capillaries have been prepared by in-situ synthesis of a macroporous polymer monolith within the capillary, which can be covalently attached to the capillary wall. Ideally, the polymerization is carried out in such a way that the capillary is not completely filled with polymer and an axial flow-pore is obtained, which allows the solvent to be exchanged easily. Entrapping imprinted polymer particles in a polyacrylamide gel formed in-situ in the capillary has been suggested as an alternative way of preparing imprinted capillaries. However, this approach seems to be somewhat less practical since the solvent cannot be exchanged easily and because the lifetime of such capillaries may be rather short due to problems with air bubble formation during operation.

Enantioselective imprinted columns for capillary electrochromatography could be very useful, especially because considerably higher efficiencies can be obtained (> 100 000 plates m"i) than with HPLC columns. Chiral separations of drugs such as the ^-adrenergic antagonist propranolol have been achieved within 2 min, and an enantiomeric mixture containing as little as 1% S-enantiomer could be resolved. Since imprinted capillaries can be prepared quickly and easily, and are normally very stable in use over a period of several months, this represents a highly promising development for analytical chiral separations.

Solar Panel Basics

Solar Panel Basics

Global warming is a huge problem which will significantly affect every country in the world. Many people all over the world are trying to do whatever they can to help combat the effects of global warming. One of the ways that people can fight global warming is to reduce their dependence on non-renewable energy sources like oil and petroleum based products.

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