Biogenic Amines Gas Chromatography

Table 1 GC of biogenic amines with FID detection

Analyte Chromato- Carrier, injector Matrix Sample treatment Reference graphic type and temper-

column flow rate ature and program

Aliphatic monoamines, aliphatic polyamines, catecholamines, indolyl amines, imidazoyl amines (57 amines)

Histamine, tyramine, putrescine, cadaverine, adrenaline, noradrenaline, tryptamine, dopamine, agmatine, spermine, spermidine, phenylethylamine

Cadaverine, 1,3-diamino-propane, putreanine, isoputreanine, putrescine, spermine, spermidine

1,3-Diaminopropane, putrescine, cadaverine, 3-aminopropylcadaverine, spermidine, sym-homo-spermidine, sym-norspermidine, spermine, sym-norspermine, thermospermine, canavalamine

Adrenaline, dopamine, noradrenaline, 5-hydroxytryptamine

Dual fused silica capillary DB-5 DB-17, length 30 m, i.d. 0.25 mm, film thickness 0.11 |im

Fused silica capillary SE-54, length 25 m, i.d. 0.31 mm, film thickness

Fused silica capillary cross-linked methyl siloxane deactivated by silanization, length 35 m,

1.d. 0.20 mm, i.d. 0.20 mm, i.d. 0.20 mm, film thickness 0.11 | m

0.5% KT-300 on Uniport HP packing, length 0.5 m,

5% OV-17 on Chromosorb W HP 80-100 mesh packing, length 1 m, i.d. 3 mm

2600C, 2600C p40Cmln"1 p 2800C

Biological fluids

Foods min

He, min


80 ml min"

36 ml mln"

1250C p 100C mln" p 3000C

3000C, 1200C p 70C mln"1 p 2600C

Biological fluids

2850C, 1300C p 100C mln"1 p 2800C


265°C isothermal

Two-phase isobutyloxy Kim etal. carbonylation, SPE (1997)

purification and deri-vatization with A/-methyl-A/-(butyldimethylsilyl)-trifluoroacetamide

Liquid—liquid extraction, Laleye etal. SPE purification, derivati- (1987) zation with trifluoroacetic anhydride

SPE purification, derivatization with heptafluorobutyrlc anhydride

Musklet etal. (1984)

Ion exchange resin separation, derivatization with ethylchloroformate

Yamamoto etal. (1984)

Biological tissues

Derivatization with pentafluorobenzoyl chloride and pyridine in acetonitrile

Bock and


Reproduced with permission by Kim KR etal. (1997).

reported to be responsible for diseases related to their biological activity.

Muskiet et al. investigated a comparison of the popular high performance liquid chromatographic (HPLC) methods, whose chief advantage is a reduced sample pre-treatment, and other chromatographic techniques, such as gas chromatography, for the separation and determination of biogenic amines. In chromatographic methods, two main steps are neces sary: (a) the separation of amines from the matrix and (b) their determination. Pre-column derivatiz-ation is very often required for selectivity and sensitivity enhancement.

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