Applications

The separation of carboxylic acids is the most common application of ion exclusion chromatography. When coupled with spectrophotometric detection at low wavelength (e.g. 210 nm), ion exclusion chromatography yields excellent separations and relatively clean chromatograms for a wide variety of complex sample matrices, such as urine, plasma, foods and beverages and pharmaceuticals. Figure 6 shows a chromatogram for a urine sample, without sample pretreatment. Ion exclusion chromatogra-phy has also found increasing use for the determination of anions of weak inorganic acids. It is especially attractive as an adjunct to ion exchange chromatog-raphy since the selectivities obtained by these two techniques are quite different. Solutes such as fluoride, carbonate, cyanide, borate and sulfite have been determined using this approach. Interference from strongly ionized species is minimal because these solutes are unretained and appear at the column void volume. Ion exclusion chromatogra-phy can therefore readily separate weakly ionized solutes in samples containing high concentrations of ionic species, e.g. sea water and oil reservoir brines.

Solar Panel Basics

Solar Panel Basics

Global warming is a huge problem which will significantly affect every country in the world. Many people all over the world are trying to do whatever they can to help combat the effects of global warming. One of the ways that people can fight global warming is to reduce their dependence on non-renewable energy sources like oil and petroleum based products.

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