Applications

None of these can be considered the application for lectin or boronated chromatography. By far the largest application of both is in the separation of glycated haemoglobin from non-glycolated haemoglobin for diagnosis of diabetes (see below).

Other diagnostic applications for both lectins and boronates have been proposed. In particular, lectins have been used to bind proteins associated with specific forms of cancer. These have the potential to be developed into useful diagnostic procedures.

In addition, both lectins and boronates have the potential to be used in large-scale purification of various glycated proteins and other glycated bio-molecules for commercial purposes. Since most ex-tracelluar mammalian proteins are glycated, and since many of these have useful commercial value, boronate chromatography is likely to develop into a significant tool for commercial purification.

Basic research also utilizes affinity chromato-graphy based on lectins and boronates. Lectins are far more commonly utilized when the goal is to identify different glycated isoforms of particular proteins or to determine which oligosaccharides are present on the surface of the cells and organelles. Utilizing successive chromatography with a bank of lectins of various specificities, coupled with glycases with distinct and varied specificities, the entire sequential composition of oligosaccharides can be readily determined.

Other uses, of course, exist for lectin and boronate chromatography; for example, the purification of specific organelles or cells utilizing the sugar moieties of glycated proteins forming the membrane surface of the organelle and/or cell.

Solar Panel Basics

Solar Panel Basics

Global warming is a huge problem which will significantly affect every country in the world. Many people all over the world are trying to do whatever they can to help combat the effects of global warming. One of the ways that people can fight global warming is to reduce their dependence on non-renewable energy sources like oil and petroleum based products.

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