Analytical Centrifugation

This is the only type of centrifugal separation in which the primary objective is not to purify or dewater one or more of the feed components. Rather, this method is used to monitor particle sedimentation behaviour. Analytical centrifugation is used to characterize particle properties such as molecular weight, diffusion and sedimentation coefficients, buoyancy density, etc. The critical component in this technique is the addition of a transparent window, e.g. quartz or sapphire, to the centrifuge rotor to permit in situ optical measurements. Sample movement is typically monitored by UV absorption or refractive index during high speed separations in ultracentrifuges. Experiments are conducted in batch mode using very small sample volumes, as low as 5 |L for some rotors. Two classes of experiments are conducted in an analytical ultracentrifugation - sedimentation velocity and sedimentation equilibrium - anlogous to rate and isopyc-nic experiments in preparative ultracentrifugation. Of these, sedimentation velocity is the more common. Analytical centrifugation is less common today than in the 1950s when this was the principal method for molecular weight determinations (1-10 kDa). However, the method is still used, primarily in biological applications, for studying phenomena such as interactions between macromolecules and ligand-induced binding events. More recently, this technique has experienced somewhat of a renaissance in drug discovery applications.

Solar Panel Basics

Solar Panel Basics

Global warming is a huge problem which will significantly affect every country in the world. Many people all over the world are trying to do whatever they can to help combat the effects of global warming. One of the ways that people can fight global warming is to reduce their dependence on non-renewable energy sources like oil and petroleum based products.

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