Affinity Precipitation

Affinity precipitation is a bioseparation technique whereby an insoluble network is formed by cross-linking a multifunctional affinity ligand with the protein of interest, thus resulting in selective precipitation of the targeted protein from solution. In principle, such a ligand should be composed of three recognition sites which are able to bind the protein simultaneously. Furthermore, the targeted protein should possess enough ligand-binding sites for the formation of a network. This technique offers certain advantages which are particularly important for large scale purification: no need for solid supports, high ligand utilization, low capital cost, and the possibility of sequential precipitation. True affinity precipitation has been demonstrated for only a few purification cases. An impressive example is the dye structure of Figure 1(F). This nonreactive molecule selectivity precipitates and purifies lactate dehydrogenase to homogeneity from crude extract of rabbit muscle. Affinity precipitation has been claimed to occur also with bis-Cibacron Blue 3GA but this technique has not found the anticipated attention.

Solar Panel Basics

Solar Panel Basics

Global warming is a huge problem which will significantly affect every country in the world. Many people all over the world are trying to do whatever they can to help combat the effects of global warming. One of the ways that people can fight global warming is to reduce their dependence on non-renewable energy sources like oil and petroleum based products.

Get My Free Ebook

Post a comment