Advantages of RPC

The advantages of the different RPC methods, can be summarized as follows:

1. Depending on the properties of the compounds to be separated, the effect of the vapour space, and thus the extent of saturation of the chromato-graphic system, can be selected freely.

2. All commercially available stationary phases can be used, irrespective of their size and quality; smaller particle size stationary phases can be used without loss of resolution because of the centrifugal force.

3. Mobile phases optimized in saturated or un-saturated analytical TLC, or in HPLC, can be transferred to the various RPC methods.

4. All three basic modes of development (circular, linear, and anticircular) and their combinations can be used for analytical separations.

5. For analytical purposes up to 72 samples can be applied to a single analytical plate, and den-sitometric quantification can be performed in situ on the plate.

6. The separation time is relatively short and scaling up to preparative methods is simple.

7. All preparative methods are online, no scratching out of the separated compounds is necessary, and the preparative separation can be recorded with a flow-through detector.

8. Because of the theoretically unlimited separation distance, the separation power can be increased

Table 1 Comparison of the different analytical and preparative FFPC (OPLC and RPC) methods

Method Viewpoint

OPLC

N-RPC

M-RPC

U-RPC

S-RPC

C-RPC

Analytical

Preparative

Preparative

Analytical

Preparative

Analytical

Preparative

Preparative

Preparative

Chamber type

Ultra-micro

Ultra-micro

Normal

Micro

Micro

Ultra-micro

Ultra-micro

Normal

Planar

column

Plate (column)

TLC/HPTLC

Pre-coated

Self-prepared

TLC/HPTLC

Self-prepared

TLC/HPTLC

Self-prepared

Self-prepared

Self-filled

pre-coated

pre-coated

pre-coated

Stationary phase

All available

Silica

Silica

All available

Silica

All available

Silica

Silica

All available

Layer thickness

0.1, 0.2 mm

0.5-2 mm

1-4 mm

0.1, 0.2 mm

1-3 mm

0.1, 0.2 mm

4 mm

1-4 mm

X= 2.24 mm

Volume of

Constant

Constant

Increasing

Constant

Increasing

Constant

Increasing

Increasing and

Constant

stationary

(increasing)

(increasing)

(increasing)

(increasing)

decreasing

phase

Particle size of

5,11 urn

5 urn < x < 25 urn

15 urn

5,11 urn

15 urn

5-11 urn

15 urn

15 urn

5 urn

stationary

phase

Separation

18 (90) cm

18 cm

10 cm

8(11) cm

10 cm

8(11) cm

10 cm

Unlimited

9 cm

distance

Separation mode

Circular, linear,

Linear (circular)

Circular

Circular, linear,

Circular

Circular, linear,

Circular

Circular and

Linear

(anticircular)

(anticircular)

(anticircular)

anticircular

Observation

Not possible

Not possible

Coloured and UV active substances can be observed during the chromatographic process

Detection

Offline, online

Online

Online

Offline

Online

Offline

Online

Online

Online

Sample number

Max. 360

1

1

max. 72

max. 72

max. 72

1

1

1

Amount of sample

ng-ng

50-500 mg

50-500 mg

ng-ng

50-500 mg

ng-ng

50-500 mg

50-500 mg

50-500 mg

significantly by employing the sequential technique.

9. On line preparative separation of 50-500 mg samples can generally be applied in a single chromato-graphic run.

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