Advantages of Chiral CCC

Countercurrent chromatography can be applied to the separation of enantiomers by dissolving a suitable chiral selector in the liquid stationary phase in analogy to binding the CS to the solid support. The HSCCC technique has the following advantages over the conventional chromatographic technique using a solid stationary phase:

1. The method permits repetitive use of the same column for a variety of chiral separations by choosing appropriate chiral selectors.

2. Both analytical and preparative separations can be performed in a standard CCC column by adjusting the amount of CS in the liquid stationary phase. The method is cost effective especially for large scale preparative separations.

3. The separation factor and peak resolution can be improved by increasing the concentration of CS in the stationary phase.

A. Collet, E cole Normale Supe rieure de Lyon, Lyon, France

Copyright © 2000 Academic Press

Methods for obtaining optically active compounds in enantiopure form are commonly classified into three categories: utilization of chiral pool starting materials (stereoselective multistep synthesis), creation of chirality from achiral precursors (asymmetric synthesis) and separation of racemates into their enan-tiomer constituents (resolution). This last method can be carried out in a variety of ways: crystallization processes, chromatography of racemates on chiral stationary phases and kinetic resolution mediated by chiral reagents or enzymes.

4. The method is very useful for investigation of the enantioselectivity of the chiral selector including determination of formation constant and separation factor.

5. pH-Zone-refining CCC can be applied to chiral separation allowing a large scale separation in a shorter separation time.

See also: II/Chromatography: Chromatography: Instrumentation. Chromatography: Liquid: Column Techonol-ogy. III/Chiral Separations: Amino Acids and Derivatives; Capillary Electrophoresis; Cellulose and Cellulose Derived Phases; Countercurrent Chromatography; Crystallization; Cyclodextrins ad Other Inclusion Complexation Approaches; Gas Chromatography; Ion-Pair Chromatography; Ligand Exchange Chromatography; Liquid Chromatography; Molecular Imprints as Stationary Phases; Protein Stationary Phases; Supercritical Fluid Chromatography; Synthetic Multiple Interaction ('Pirkle') Stationary Phases; Thin-Layer (Planar) Chromatography. Zone Refining Countercurrent Chromatography.

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