AAGe RTln a RTln [KK

A stationary phase that produces a large degree of separation is said to have a high selectivity. The separation of two components (1 and 2) is expressed by the relative retention (a):

a " tR(2)/tR(1) = VR(2)/VR(1) = k(2)/k(1) = KC(2)/KC(1)

If one of the pair is a standard substance, the symbol used for relative retention is 'r'.

Having achieved a separation it is necessary to prevent remixing of the components. The ability to achieve this is a function of the column efficiency, as measured by the plate number. The combined effects of stationary phase selectivity and column efficiency is expressed in the peak resolution (Rs) of the column. (see Figure 2):

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Solar Panel Basics

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