24Dinitrophenylhydrazone Derivatization

2,4-Dinitrophenylhydrazone (DNPH) derivatives of aldehydes and ketones have been used in gas chromatography for many years. The reaction procedure of aldehyde or ketone is as follows:

Kallio et al. analysed 15 carbonyl compounds (aldehydes and ketones) known to be Savour components by derivatization/GC with DNPH. The DNPHs of the carbonyl compounds were prepared by shaking 100 |L of each compound with 100 mL of a saturated solution of DNPH in aqueous 2molL_1 hydrochloric acid and allowing the mixture to stand at room temperature overnight. The precipitated DNPHs were dissolved in ethyl acetate, then analysed by GC-FID or dissolved in benzene and analysed by GC-ECD (electron-capture detector). Packed columns with silicone stationary phases were used. Relative retention times of DNPHs of aldehydes and ketones on one of these columns are listed in Table 1

Solar Panel Basics

Solar Panel Basics

Global warming is a huge problem which will significantly affect every country in the world. Many people all over the world are trying to do whatever they can to help combat the effects of global warming. One of the ways that people can fight global warming is to reduce their dependence on non-renewable energy sources like oil and petroleum based products.

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