24Dinitrophenylhydrazone Derivatization

2,4-Dinitrophenylhydrazone (DNPH) derivatives of aldehydes and ketones have been used in gas chromatography for many years. The reaction procedure of aldehyde or ketone is as follows:

Kallio et al. analysed 15 carbonyl compounds (aldehydes and ketones) known to be Savour components by derivatization/GC with DNPH. The DNPHs of the carbonyl compounds were prepared by shaking 100 |L of each compound with 100 mL of a saturated solution of DNPH in aqueous 2molL_1 hydrochloric acid and allowing the mixture to stand at room temperature overnight. The precipitated DNPHs were dissolved in ethyl acetate, then analysed by GC-FID or dissolved in benzene and analysed by GC-ECD (electron-capture detector). Packed columns with silicone stationary phases were used. Relative retention times of DNPHs of aldehydes and ketones on one of these columns are listed in Table 1

Solar Panel Basics

Solar Panel Basics

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