## Spe

Of the batch, the contribution plot for SPE signals an unusual situation for variable 3 (Figure 6.80c). Variable 3 and 11 are found to be the most affected variables because of the fault according to T2 contribution plot. Deviation from average batch behavior plot is ineffective in indicating the most affected variable(s) in this case (Figure 6.83a). Quality prediction ability of the integrated MSPM framework is also tested via two cases. A normal batch is investigated first. As expected, SPE...

## 272 A Structured Model for Penicillin Production

The model proposed in this case is a derivative of the morphologically structured model by Nielsen 424 and accounts for effects of dissolved oxygen on cell growth and penicillin production and variations in volume fractions of abiotic and biotic phases due to biomass formation 63 . Penicillin production is considered to occur in the subapical hyphal cell compartment and to be affected by glucose and oxygen. The morphological structure of the model is described in detail elsewhere 423, 424 ....

## Dx

A linear time-varying global model is constructed by using the local models to approximate the nonlinear dynamics of a batch run. The time-varying model is obtained by interpolating between local models by using model validity functions Pi(t) which are similar to the interpolation function u> i of Eq. 4.114. Model validity functions are the estimates of the validity of various local models in different operating points and for N local models p(t) pi(t),p2(t),--- ,pN(t) > i(t) 1- (4-118) The...

## Chemical Industries

A Series of Reference Books and Textbooks 1. Fluid Catalytic Cracking with Zeolite Catalysts, Paul B. Venuto and E. Thomas Habib, Jr. 2. Ethylene Keystone to the Petrochemical Industry, Ludwig Kniel, Olaf Winter, and Karl Stork 3. The Chemistry and Technology of Petroleum, James G. Speight 4. The Desulfurization of Heavy Oils and Residua, James G. Speight 5. Catalysis of Organic Reactions, edited by William R. Moser 6. Acetylene-Based Chemicals from Coal and Other Natural Resources, Robert J....

## Cmg

Figure 7.4. (a) Operating diagram for Example 1 with the cell growth following Monod kinetics (pm 1.0 h l, K 0.05 gL-1, and Kj > oo in Table 3, D in h l and SF in gL l). p(SF) D on the curve ABODE. Forced periodic operation with variations in D and Sp is superior to steady-state operation (i) at all frequencies in region I (Spo, Do) lying below the curve ABFG , (ii) for 0 < u) < wi and U2 < u> < oo (u> 2 > u> i) in region II (Spo, D0) lying between the curves BFG and BCDE ,...

## Yt Ctxt Ct yt ytyr 7106

A t , B t , C t and E i are the appropriately dimensioned system matrices with the respective multiplying vectors, the elements of which are partial derivatives evaluated at the reference state xr, ur, dr . If the reference state happens to be a steady state admissible only in a continuous bioreactor operation , then the system matrices are time-invariant. In that case, the state-space representation in Eqs. 7.104 and 7.106 can be transformed into transfer function representation by applying...

## 25 Unstructured Kinetic Models

An unstructured kinetic representation provides a simplistic global with respect to cell mass view of the net result of metabolic rate processes occurring in the living cells. In brief, the unstructured representation can be described as Nutrients 2 Ni Cell mass 2-16 more Cell mass Non-biomass Products Pj This representation then involves conservation equations for cell mass, key nutrients, and metabolites of interest target products , the rates of generation consumption of the individual...

## 332 Fractional Factorial Design

The number of experiments required in a full 2fc factorial design increases geometrically with k. For a process with 7 factors, for example 27 128 experiments are needed to estimate the 7 main effects, and 120 interactions. There are 21 two-factor, 35 three-factor, 35 four-factor, 21 five-factor, 7 six-factor and 1 seven-factor interactions 78 . Fortunately, not all of these interactions are important. Furthermore, the main effects tend to be larger in absolute magnitude than the two-factor...

## 271 An Unstructured Model for Penicillin Production

In this case study, the mechanistic model of Bajpai and Reuss 36 was used as starting point for model development. The original model has been extended by including additional input variables such as agitation power, and aeration rate. Functional relationships among the process variables are summarized in Table 2.3 and all inputs and outputs are listed in Figure 2.1. A variety of mathematical representations have been suggested for describing certain biological behaviors by researchers...

## 33 Statistical Design of Experiments

Experiments are frequently performed to assess the effects of inputs, operating conditions, and changes in the process on the outputs. For example, the effects of variations in fermentation temperature, air flow rate, or strain type used on the attributes of a product would provide valuable information for optimizing productivity. Experiments are costly since they consume time and raw materials. Properly planned experiments minimize unnecessary duplications, generate more information with fewer...

## I

Downstream processes in industrial scale penicillin production. culture volume, sequential growth is followed in this manner. Inoculum size is typically around 10 of the total culture volume. Since formation of secondary metabolites in this case, penicillin is usually not associated with cell growth, it is a common practice to grow the cells in a batch culture followed by a fed-batch operation to promote synthesis of the antibiotic. When inoculum at the desired concentration is...